Alain Garnier, ing.

Professeur titulaire

Alain Garnier collabore, avec des équipes pluridisciplinaires, au développement de thérapies cellulaires contre des maladies incurables et contribue également à la conception de nouvelles technologies biologiques vertes et durables.

Champs d'intérêts

  • Bioingénierie
  • Conception, développement, optimisation et mise à l’échelle de bioprocédés
  • Production de métabolites, protéines recombinantes, vecteurs viraux, vaccins et cellules thérapeutiques
  • Ingénierie des cultures cellulaires
  • Bioprocédés environnementaux, énergétiques, verts et durables
Alain Garnier, professeur au Département de génie chimique

  • GCH-2100 Génie biochimique II
  • GCH-2105 Assainissement industriel
  • GCH-2005 Cinétique et catalyse
  • GCH-2006 Calcul des réacteurs chimiques
  • GCH-6000 Communication scientifique orale et écrite I
  • GCH-7003 Cinétique biochimique
  • GCH-8001 Communication scientifique orale et écrite II

  • V. K. Lazarjan, A. B. Gashti, M. Feshki, A. Garnier and B. Gosselin. (2021). "Miniature Fiber-Spectrophotometer for Real-Time Biomarkers Detection". IEEE Sensors J. 21(13): 14822-14837.
  • Rasouli H, Iliuta I, Bougie F, Garnier A, Iliuta MC. (2021). "Enzyme-immobilized flat-sheet membrane contactor for green carbon capture". Chem Eng J. 421(1): 129587
  • Vincent T, Gaillet B, Garnier A. (2021). "Optimization of operation conditions for improved cytochrome P450BM3 enzymatic reaction yield". Can J Chem Eng. Online preview
  • Vincent T, Gaillet B, Garnier A (2021), "Optimisation of Cytochrome P450 BM3 Assisted by Consensus-Guided Evolution". Appl Biochem Biotechnol, early access, DOI: 10.1007/s12010-021-03573-1.
  • Awad G, Garnier A (2021), "Maximization of saturated fatty acids through the production of P450BM3 monooxygenase in the engineered Escherichia coli". Food Bioprod Process, 126:130-142.
  • Awad G, Garnier A (2021), "Promising optimization of bacterial cytochrome P450BM3 enzyme production by engineered Escherichia coli BL21". Biocat Agric Biotechnol, 31:101917. DOI: 10.1016/j.bcab.2021.101917.
  • Mangion M, Gelinas JFGashti ABZ Azizi H, Kiesslich S, Nassoury N, Chahal P, Kobinger G, Gilbert R, Garnier A, Gaillet B, Kamen A (2020), "Evaluation of novel HIV vaccine candidates using recombinant vesicular stomatitis virus vector produced in serum-free Vero cell cultures". Vaccine, 38(50):7949-7955.
  • Azizi D, Larachi F, Garnier A, Lague P, Levasseur B (2020), "Sorption of Aqueous Amino Acid Species on Sulfidic Mineral Surfaces – DFT Study and Insights on Biosourced-Reagent Mineral Flotation". Can J Chem Eng, 99(8):1758-1779.
  • Bahrami A, Iliuta I, Garnier A, Larachi F, Vincent V, Iliuta MC (2019):"Kinetics of Enzymatic Hydroxylation by Free and MNPs-Immobilized NADH-Dependent Cytochrome P450 BM3 from Bacillus megaterium". Ind Eng Chem Res, 58(2):808-815.
  • Laberge A, Ayoub A, Arif S, Larochelle S, Garnier A, Moulin VJ (2019), "α-2-Macroglobulin induces the shedding of microvesicles from cutaneous wound myofibroblasts". J Cell Phys, 234(7):11369-11379.
  • Hoesli CATremblay CJuneau PMBoulanger MD, Beland AV, Ling SD, Gaillet B, Duchesne C, Ruel J, Laroche G, Garnier A (2018), "Dynamics of Endothelial Cell Responses to Laminar Shear Stress on Surfaces Functionalized with Fibronectin-Derived Peptides". ACS Biomater Sci Eng, 4(11):3779-91.
  • Bahrami AGarnier ALarachi FIliuta, MC (2018), "Covalent immobilization of cytochrome P450 BM3 (R966D/W1046S) on glutaraldehyde activated SPIONs". Can J Chem Eng, 96: 2227-2235.
  • Ghio SC, Larouche D, Garnier A, Germain L (2018), “Development of a keratinocyte-specific defines media suitable for self-assembled bilayered skin substitute production”. Cytotherapy, 20(5):S85-S86.
  • Del Guidice T, Lepetit-Stoffaes JP, Bordeleau LJ, Roberge J, Theberge V, Lauvaux C, Barbeau X, Trottier J, Dave V, Roy DC, Gaillet B, Garnier A, Guay D (2018), Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells”. Plos One, 13(4). DOI: 10.1371/journal.pone.0195558
  • Bahrami, A, Vincent, T, Garnier, A, Larachi, F, Boukouvalas, J, Iliuta, M (2017), “Non-covalent immobilization of optimized bacterial cytochrome P450 BM3 on functionalized magnetic nanoparticles”. Ind Eng Chem Res, 56(39):10981-10989.
  • Juneau PM, Garnier A, Duchesne C (2017), "Monitoring of adherent live cells morphology using the Undecimated Wavelet Transform Multivariate Image Analysis (UWT-MIA)". Biotechnol Bioeng, 114(1):141-153.
  • Ouellet, DL, Rousseau, J, Cherif, K, Gerard, C, Gilbert, R, Garnier, A, Gaillet, B, Beauséjour, C, Tremblay, JP. (2016). "Deletion of Mutated GAA Repeats from the Intron 1 of the Frataxin Gene Using the CRISPR System Restores the Protein Expression in a Friedreich Ataxia Model. Molecular Therapy. 24: S129-S129.
  • Tremblay-Bouliane, K., Tremblay, D., Moineau, S., Gaillet, B., Garnier, A. (2016), "Screening peptide-ore interactions with a phage display library for bioflotation application". CIM J, 7 (3).
  • Parent, V.A., Tremblay, J.P., Garnier, A. (2016), "Rational design of a serum-free culture medium for the growth of human myoblasts destined to cell therapy". Can. J. Chem. Eng., 94(10):1855–1862.
  • Brassard JA, Fekete N, Garnier A, Hoesli, CA (2016), " Hutchinson-Gilford progeria syndrome as a model for vascular aging". Biogerontol, 17(1):129-145.
  • Iliuta I, Garnier A, Iliuta MC (2015), "Enzymatic Fatty Acid Hydroxylation in a Liquid–Liquid Slug Flow Microreactor". Ind Eng Chem Res, 54:7787-7799.
  • Garnier A, Gaillet B (2015), "Analytical Solution of Luedeking-Piret Equation for a Batch Fermentation Obeying Monod Growth Kinetics". Biotechnol Bioeng, 112(12):2468-2474.
  • Juneau PM, Garnier A, Duchesne C (2015), "The Undecimated Wavelet Transform-Multivariate Image Analysis (UWT-MIA) for simultaneous extraction of spectral and spatial information”. Chemom. Int Lab Syst, 142:304-318
  • Hoesli CA, Juneau PM, Chevallier P, Duchesne C, Garnier A, Laroche G (2014), "A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces". Biomaterials, 35(3), 879-890.
  • Segura MM, Mangion M, Gaillet B, Garnier A (2013), "New developments in lentiviral vector design, production and purification". Expert Opin Biol Ther, 13(7):987-1011.
  • Juneau PM, Garnier A, Duchesne C (2013), "Selection and tuning of a fast and simple phase-contrast microscopy image segmentation algorithm for measuring myoblast growth kinetics in an automated manner". Microsc Microanal, 19(4), 855-866.
  • Michaud FT, Havugimana PC, Duchesne C, Sanschagrin F, Bernier A, Lévesque RC, Garnier A (2012), "Cell culture tracking by multivariate analysis of raw LCMS data". Appl Biochem Biotechnol, 167(3):474-488.
  • Leysi-Derilou Y, Duchesne C, Garnier A, Pineault N (2012), "Single Cell Level Analysis of Megakaryocyte Growth and Development". Differentiation, 83:200-209.
  • Robert A, Cortin V, Garnier A, Pineault N (2012), "Megakaryocyte and platelet production from human cord blood stem cells". Methods Mol Biol, 788:219-247.
  • Pineault N, Cortin V, Boyer L, Garnier A, Robert A, Thérien C, Roy DC (2010), "Individual and synergistic cytokine effects controlling the expansion of cord blood CD34+ cells and megakaryocyte progenitors in culture". Cytotherapy, 13(4):467-480.
  • Gaillet B, Gilbert R, Broussau S, Pilotte A, Malenfant F, Mullick A, Garnier A, Massie B (2010), "High-level recombinant protein production in CHO cells using lentiviral vectors and the cumate gene-switch". Biotechnol Bioeng, 106(2):203-215.
  • Leysi-Derilou Y, Robert A, Duchesne C, Garnier A, Boyer L, Pineault N (2010), « Polyploid megakaryocytes can complete cytokinesis ». Cell Cycle, 9(13):2587-2597.  
  • Caron AW, Nicolas C, Gaillet B, Ba I, Pinard M, Garnier A, Massie B, Gilbert R (2009), "Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins". BMC Biotechnol, 9:42-52.
  • Michaud, FT, Garnier, A, Lemieux, L, Duchesne, C (2008), "Multivariate analysis of single quadrupole LCMS spectra for routine characterization and quantification of intact proteins". Proteomics Proteomics, 9 (3):746-756.
  • Segura MM, Garnier A, Di Falco MR, Whissell G, Meneses-Acosta A, Arcand N, Kamen A (2007), " Identification of host proteins associated with  retroviral vector particles by proteomic analysis of highly purified vector preparations". J Virol, 82:1107-1117.
  • Segura MM, Garnier A, Durocher Y, Coelho H, Kamen A (2007), "Production of Lentiviral Vectors by Large-Scale Transient Transfection of Suspension Cultures and Affinity Chromatography Purification". Biotechnol Bioeng, 98 (4), 789-799.
  • Gilbert PA, Kamen A, Bernier A, Garnier A (2007), "A simple macroscopic model for the diffusion and adsorption kinetics of r-adenovirus". Biotechnol Bioeng., 98:239:251.
  • Gaillet B, Gilbert R, Amziani R, Guilbault C, Gadoury C, Mullick A, Garnier A, Massie, B (2007), "High-level recombinant protein production in CHO cells using an adenoviral vector and the cumate gene-switch". Biotechnol Prog, 23(1):200-209.
  • Paradis-Bleau C, Cloutier I, Lemieux L, Sanschagrin F, Laroche J, Auger M, Garnier A, Levesque R (2007), "Peptidoglycan lytic activity of the Pseudomonas aeruginosa phage PhiKZ gp144 lytic transglycosylase". FEMS Microbiol Lett, 266:201-209.
  • Segura MM, Kamen A, Lavoie M-C, Garnier A (2007), "Exploiting heparin-binding properties of MoMLV-based retroviral vectors for affinity chromatography". J Chrom B, 846:124-131.
  • Ghani K, Garnier A, Coelho H, Transfiguracion J, Trudel P, Kamen A (2005), "Retroviral vector production using suspension-adapted 293GPG cells in a 3L acoustic filter-based perfusion bioreactor". Biotechnol Bioeng, 95(4):653-660.
  • Segura, MM, Garnier, A, Kamen, A (2006), "Purification and characterization of retrovirus vector particles by rate zonal ultracentrifugation". J Virol Methods, 133:82-91.
  • Segura MM, Kamen A, Garnier A (2005), "Downstream processing of oncoretroviral and lentiviral gene therapy vectors". Biotechnol Adv, 24(3):321-337.
  • Segura, MM, Kamen, A, Trudel, P, Garnier, A (2005), "A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography". Biotechnol Bioeng, 90(4):391-404.
  • Michaud, FT, Parent, V, Garnier, A, Duchesne, C (2005), "On the Proper Use of Statistical Design of Experiments". Exp Hematol, 33(11):1271-1272.
  • Cortin, V, Garnier, A, Pineault, N, Boyer, L, Lemieux, R, Proulx, C (2005), "Efficient In Vitro Megakaryocyte Maturation Using Cytokine Cocktails Optimized by Statistical Experimental Design". Exp Hematol, 33 (10): 1182-1191.
  • Cortin, V, Thibault, J, Jacob, D, Garnier, A (2004), "Recombinant High-titer adenovirus vector production in 293S cell perfusion culture." Biotechnol Progress, 20(3):858-63.
  • Jardon, M, Garnier, A (2003), "Effect of pH, PCO2, and Temperature on Recombinant Adenovirus Production". Biotechnol Prog, 19:202-208.
  • Boudreault, P, Tremblay, JP, Pépin, MF, Garnier, A. (2001), "Scale-Up of a Myoblast Cell Culture Process". J. Biotechnol., 91 (1):63-74.
  • El Zoeiby, A, Sanschagrin, F, Havugimana, PC, Garnier, A, Lévesque, RC (2001), "In vitro reconstruction of the biosynthetic pathway of peptidoglycan cytoplasmic precursor in Pseudomonas aeruginosa". FEMS Microbiol Lett, 201(2):229-235.
  • Pouliot, K, Thibault, J, Garnier, A, Acuna G (2000), "KLa evaluation during the course of a fermentation using data reconciliation techniques". Bioproc Eng, 23(6), 565-573.
  • Gilbert, PA, Garnier, A, Jacob, D, Kamen, A (2000), "On-line measurement of GFP fluorescence for the monitoring of recombinant adenovirus production". Biotechnol Letters, 22:561-567.
  • Côté, J, Garnier, A, Massie, B, Kamen, A (1998), “Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells”. Biotechnol Bioeng, 59(5):567-575.
  • Côté, J, Bourget, L, Garnier, A, Kamen, A (1997), “Study of adenovirus production in serum-free 293SF suspension culture by GFP-expression monitoring”. Biotechnol Progress, 13(6):709-714.
  • Jani, A, Lochmuller, H, Acsadi, G, Simoneau, M, Huard, J, Garnier, A, Karpati, G, and Massie, B (1997), "Generation, validation and large scale production of adenoviral recombinants with large size inserts such as a 6.3 kb human dystrophin cDNA". J Virol Meth, 64:111-124.
  • Ramsay, J, Rhazi, B, Garnier, A, Sapieha, S, Chavarie, C, Ramsay, B (1997), "Attachment of Vero cells to corona-treated, plastic surfaces". J Ferm Bioeng, 83:173-178.
  • Nadeau, I, Garnier, A, Côté, J, Massie, B, Chavarie, C, Kamen, A (1996), "Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies". Biotechnol Bioeng, 51(6): 613-623.
  • Garnier, A, Voyer, R, Tom, R, Perret, S, Jardin B, Kamen, A (1996), "Dissolved carbon dioxide accumulation in a large scale and high density production of TGF  receptor with baculovirus infected Sf-9 cells". Cytotechnology, 22: 53-63.
  • Kamen, A, Garnier, A, André, G, Archambault, J, Chavarie, C (1995), "Determination of mass transfer parameters in surface aerated bioreactors with bubble entrainment". Chem Eng J, 59: 187-193.
  • Garnier, A, Côté, J, Nadeau, I, Kamen, A, Massie, B (1994), "Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells". Cytotechnology, 15: 145-155.
  • Chavarie, C, Karamanev, D, Godard, F, Garnier, A, André, G (1993): "Comparison of the Growth Kinetics between Three Different Strains of Thiobacillus Ferrooxidans on Ferrous Sulfate". Geomicrobiology J, 11: 57-63.
  • Garnier, A, Chavarie, C, André G, Klvana, D (1990), "The inverse fluidization airlift bioreactor, part I: Hydrodynamic studies". Chem Eng Comm, 98: 31-45.

  • Membre de l’Ordre des ingénieurs du Québec (OIQ)
  • Laboratoire d'optimisation des bioprocédés (LOB)
  • Réseau canadien de centres d'excellence sur les cellules souches (STEMCELLNET, RCE)
  • Réseau de thérapie cellulaire et tissulaire (ThéCell, FRSQ)
  • Association de thérapie génique du Québec (ATGQ)
  • Centre de recherche PROTEO (FRNTQ)
  • Laboratoire d’observation et d’optimisation des procédés (LOOP)
  • Société canadienne de génie chimique (CSChE)
  • American Institute of Chemical Engineering (AIChE)
  • American Chemical Society (ACS)

  • Ph.D. génie chimique, École Polytechnique de Montréal, 1992
  • M.Sc. génie chimique, École Polytechnique de Montréal, 1987
  • B. Ing., École Polytechnique de Montréal, 1985